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(EurekAlert)   Got massive steppe migration?   (eurekalert.org) divider line
    More: Interesting, Bronze Age, Horse, Domestication of the horse, Eurasian nomads, Milk, Eurasian steppe, Bronze Age pastoralists, Livestock  
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842 clicks; posted to STEM » on 16 Sep 2021 at 3:20 PM (4 weeks ago)   |   Favorite    |   share:  Share on Twitter share via Email Share on Facebook



13 Comments     (+0 »)
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2021-09-16 1:19:52 PM  
Not yet but we will once global warming ramps up to 11.
 
2021-09-16 2:40:27 PM  
But they migrated because where they ended up was a steppe up from where they came from.

/they walked there steppe by steppe
 
2021-09-16 3:54:37 PM  
I googled steppe sister movies and was sent here. I think I misspelled something.
 
2021-09-16 4:01:22 PM  
So, milk is to blame for the plague then?
 
2021-09-16 4:59:33 PM  

Combustion: But they migrated because where they ended up was a steppe up from where they came from.

/they walked there steppe by steppe


"Ray, the steppe migrated about a foot and a half."
 
2021-09-16 5:24:23 PM  
Old news. We've known for a long time that milk and cheese fed the armies of Ghengis Khan.
 
2021-09-16 5:27:22 PM  

wax_on: Old news. We've known for a long time that milk and cheese fed the armies of Ghengis Khan.


Fark user imageView Full Size
 
2021-09-16 6:46:35 PM  
the actual paper describes long involved sample handling and preparation.
I stumbled across this paragraph.

https://www.nature.com/articles/s4158​6​-021-03798-4
Protein extraction
For samples with a 'Z' designation, proteins were extracted using a modified low-volume filter-aided sample preparation that has previously been described35. To decrease contamination, 1 ml of 0.5 M EDTA was then added to each sample tube, samples were rotated for 5 min followed by centrifugation at 20,000 rcf for 10 min to remove the any contamination on the outer layer of calculus; the supernatant was then removed and retained. Then, 1 ml of 0.5 M EDTA was added to each decontaminated sample, and the sample was allowed to decalcify on a rotator for 5-7 days at room temperature until completion. After demineralization, samples were centrifuged at top speed (20,000 rcf) for 5 min. Eight hundred μl of EDTA supernatant was removed and stored for future analysis. Separately, 50 μl of urea solution (8 M) was added to a 30-kDa Millipore Microcon filter unit. Samples were denatured, reduced and alkylated with 30 μl of sodium dodecyl sulfate (SDS)-lysis buffer (4% w/v SDS, 100 mM Tris/HCL pH 8.2 and 0.1 M DTT), incubated at 95°C for 5 min, and 100 μl of iodoacetamide (IAA) solution (0.5 M IAA and 8 M UA) was added to the filter units and was mixed at 600 rpm for 1 min in the dark. Following incubation, the samples were centrifuged at 14,000g for 10 min. Two hundred μl of UA (8 M) was added to the filter unit, followed by the lysed sample supernatant and units centrifuged for 20 min at 14,000g. UA was used twice to remove the IAA and followed by centrifugation for 12-15 min at top speed. One hundred μl of 0.5 M NaCl was added to each filter unit, spun for 12 min at 14,000g. This step was repeated and the flowthrough discarded. The filter units were transferred to new units, and 120 μl of trypsin solution (3 μl of 0.4 μg μl−1 trypsin in 117 μl of 0.05 M triethylammoniumbicarbonate) was added to each unit. Units were thermomixed at 600 rpm for 1 min and then incubated overnight at 37°C. Following digestion, samples centrifuged for 20 min at 14,000g and acidified with 5% TFA to a pH of <2.


ummm why was this stuff "mixed at 600 rpm for 1 min in the dark"?
 
2021-09-16 7:18:00 PM  
Milk: It Does a Mongol Horde Good.
 
2021-09-16 8:41:42 PM  
biography.comView Full Size

Pleased to help
 
2021-09-16 10:34:27 PM  
You can have your cow and eat it too.
 
2021-09-17 2:18:42 AM  

Myk-House of El: wax_on: Old news. We've known for a long time that milk and cheese fed the armies of Ghengis Khan.

[Fark user image 600x931]


But what about milk and cookies?

Fark user imageView Full Size


/it was the 90s, you see...
 
2021-09-17 3:20:39 AM  

HairBolus: ...

ummm why was this stuff "mixed at 600 rpm for 1 min in the dark"?


Don't judge them too hard.  As you get older it simply becomes more difficult and complicated to achieve arousal.
 
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