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(Science Magazine)   Ok stem cells are coming out   (news.sciencemag.org) divider line 9
    More: Interesting, stem cells, tissue engineering, RIKEN, cell type, pluripotent stem cells, Chinese University of Hong Kong  
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2606 clicks; posted to Geek » on 24 Mar 2014 at 9:13 PM (25 weeks ago)   |  Favorite    |   share:  Share on Twitter share via Email Share on Facebook   more»



9 Comments   (+0 »)
   
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2014-03-24 10:06:57 PM
Whoa. "Acquisition of Pluripotency" was my band's name in 1987.
 
2014-03-24 10:07:50 PM
But it didn't work?
 
ZAZ [TotalFark]
2014-03-24 10:08:22 PM
The Vacanti lab's technique includes what they call an "extremely important step" that was not described in the Nature paper or updated protocol published earlier: forcing the mature cells through very small pipettes for at least 25 minutes before exposing them to the acid solution.

Biology workers should record video of their experiments, end to end, so they can catch such omissions. Chant "hail Mary." Use distilled water instead of tap water. Have the really strong guy tighten the lid. Whatever.

Alternatively, have a lab rat undergrad do the experiment from the written protocol and see what goes wrong. I remember learning this around first grade when a teacher had me follow my own instructions, which missed a critical step.  (She prompted me when I started doing something I hadn't put in the instructions because it was so obvious.)
 
2014-03-24 10:21:05 PM

ZAZ: The Vacanti lab's technique includes what they call an "extremely important step" that was not described in the Nature paper or updated protocol published earlier: forcing the mature cells through very small pipettes for at least 25 minutes before exposing them to the acid solution.

Biology workers should record video of their experiments, end to end, so they can catch such omissions. Chant "hail Mary." Use distilled water instead of tap water. Have the really strong guy tighten the lid. Whatever.

Alternatively, have a lab rat undergrad do the experiment from the written protocol and see what goes wrong. I remember learning this around first grade when a teacher had me follow my own instructions, which missed a critical step.  (She prompted me when I started doing something I hadn't put in the instructions because it was so obvious.)


Check out JoVE. It's sweet.
 
2014-03-24 10:44:07 PM
Boomer sooner!!
 
2014-03-24 11:45:00 PM
I have seen a few Gianna Michaels porn clips end this way.
 
2014-03-24 11:53:38 PM
So, creationist are the douches in this case?
 
2014-03-25 03:41:59 AM
This whole thing is mystifying. Why they would publish this paper without verifying the result, and on top of that leave out a step like that in the description of the method.

Sloppy.
 
2014-03-25 12:34:38 PM
What a stupid use of this meme (which is already fairly stupid).
 
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