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(Popular Science)   "Wow, Timmy, looks like Santa brought you you very own home DNA replicator device. Doesn't that look like fun?"   (popsci.com) divider line 19
    More: Strange, DNA, polymerase chain reaction, base pairs, nucleotides, microcontrollers, reagents, adapters, heredity  
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1768 clicks; posted to Geek » on 15 May 2013 at 8:54 AM (1 year ago)   |  Favorite    |   share:  Share on Twitter share via Email Share on Facebook   more»



19 Comments   (+0 »)
   
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2013-05-15 08:07:33 AM
Doesn't that sound like fun? Timmy? Oh....very funny. Which one of you is the real Timmy?
 
2013-05-15 09:05:55 AM
I got yer DNA replicator right here.
 
2013-05-15 09:07:48 AM
OK, so a temperature-controlled chamber and " You'll need to order from the Internet reagents that can hijack DNA-copying molecules ".

Yeah, wow.
 
2013-05-15 09:25:37 AM
That's the same present every boy gets around the time he turns 13 or so
 
2013-05-15 09:28:16 AM
This is a scam. They're only advertising a do-it-yourself PCR machine so they can make the real money on the back end: selling agarose and ethidium bromide to gullible consumers.
 
2013-05-15 09:45:06 AM
24.media.tumblr.com
 
2013-05-15 09:46:12 AM
FTA:  Professional machines cost about $10,000 dollars each

Like nails on a chalkboard.  Are there no editors anymore?
 
2013-05-15 10:27:13 AM
Quick, save the website offline before ARM the Feds make it disappear!

/will be obscure to someone
 
2013-05-15 10:42:18 AM
Never have I believed in a more malevolent and destructive god than when I was doing PCR day in, and day out. That machine would fail for reasons like a fly farted on it too hard. I swear.
 
2013-05-15 11:23:41 AM
Hmm.  I've got all the required parts (sans reagents) on hand already.  I'd probably use nichrome wire instead of a light bulb, though.  Also solid state relays instead mechanical ones;  they're silent and can be pulse modulated.
 
2013-05-15 12:34:24 PM

Frozboz: FTA:  Professional machines cost about $10,000 dollars each

Like nails on a chalkboard.  Are there no editors anymore?


I'm going to use my PIN number at the ATM machine to withdraw that $10,000 dollars.
 
2013-05-15 12:35:38 PM

RatOmeter: Hmm.  I've got all the required parts (sans reagents) on hand already.  I'd probably use nichrome wire instead of a light bulb, though.  Also solid state relays instead mechanical ones;  they're silent and can be pulse modulated.


Check eBay for "Sestos" temp controllers. They work well. I got one to futz around with my espresso maker.

www.sestos-hk.com

With thermocouple (I forget the type, K?) and SSR.

This is fantastic, you just plug it all to the mains and it just works. I don't particularly feel like debugging some amateur's PID loop on an Arduino.
 
2013-05-15 12:57:36 PM
Can I use it to frame others for my crimes?
 
2013-05-15 01:08:34 PM

Quantum Apostrophe: RatOmeter: Hmm.  I've got all the required parts (sans reagents) on hand already.  I'd probably use nichrome wire instead of a light bulb, though.  Also solid state relays instead mechanical ones;  they're silent and can be pulse modulated.

Check eBay for "Sestos" temp controllers. They work well. I got one to futz around with my espresso maker.

www.sestos-hk.com

With thermocouple (I forget the type, K?) and SSR.

This is fantastic, you just plug it all to the mains and it just works. I don't particularly feel like debugging some amateur's PID loop on an Arduino.


Hehehehe...reminds me...I have a spare Watlow for the curing over I could play with. :^)

www.watlowdistributor.com
 
2013-05-15 03:18:05 PM

Stone Meadow: Quantum Apostrophe: RatOmeter: Hmm.  I've got all the required parts (sans reagents) on hand already.  I'd probably use nichrome wire instead of a light bulb, though.  Also solid state relays instead mechanical ones;  they're silent and can be pulse modulated.

Check eBay for "Sestos" temp controllers. They work well. I got one to futz around with my espresso maker.

[www.sestos-hk.com image 400x400]

With thermocouple (I forget the type, K?) and SSR.

This is fantastic, you just plug it all to the mains and it just works. I don't particularly feel like debugging some amateur's PID loop on an Arduino.

Hehehehe...reminds me...I have a spare Watlow for the curing over I could play with. :^)

[www.watlowdistributor.com image 288x252]


I've got one for suos vide, but I'm not sure how important tight regulation is.  His Arduino code doesn't do any fancy closed loop:

1) turn heat on
2) shut off heat at a certain temp
3) dwell for a while
4) turn on fan
5) shut off fan at a certain lower temp
6) dwell for a while
7) turn heat on
8) shut off heat at a certain not-quite-so high temp
9) dwell for a while
10) goto 1

He does note that maybe he should maintain that last temp during the dwell in step 9, rather than let it decay.  So it sounds like maybe the process could be improved with a proper PID.  I'll have to read up on PCR.
 
2013-05-15 04:45:53 PM
I don't see the polymerase, buffers, or primers in that $50 supply list.
 
2013-05-15 05:24:47 PM
the idea of a big light bulb emitting UV right next to my DNA seems kinda dumb to me.
 
2013-05-15 05:58:47 PM

coffemonster: the idea of a big light bulb emitting UV right next to my DNA seems kinda dumb to me.


Er, incandescent lamps emit a negligible amount of UV.  Fluorescent lamps put out more UV, but a lot less heat - and heat is the point of the lamp in this device.  If you insist that UV would be a problem, then use another heat source, like a heater made of nichrome wire as I mentioned above.

Another mod I'd consider to the linked design is to add a reflective material such as aluminum duct tape to the ID of the pipe.  Cheap, simple and might help the PVC pipe from getting as hot (though with the tape stuck directly on the pipe, there will be thermal conduction).
 
2013-05-16 01:22:59 AM

abhorrent1: Can I use it to frame others for my crimes?


This actually came up in an Israeli court case. No. Your DNA contains methylation cues and other epigenitc signals that a PCR product simply lacks. If you're a good geneticist, you can tell the difference between a manufactured strand and a natural strand. It's not a huge difference, but big enough to where you can distinguish them.
 
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